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Leachianone A
| Product Name | Leachianone A |
| Price: | $234 / 5mg |
| Catalog No.: | CN05213 |
| CAS No.: | 97938-31-3 |
| Molecular Formula: | C26H30O6 |
| Molecular Weight: | 438.5 g/mol |
| Purity: | >=98% |
| Type of Compound: | Flavonoids |
| Physical Desc.: | Powder |
| Source: | The roots of Sophora flavescens. |
| Solvent: | Chloroform, Dichloromethane, Ethyl Acetate, DMSO, Acetone, etc. |
| SMILES: | COc1cc(O)ccc1[C@@H]1CC(=O)c2c(O1)c(C[C@H](C(=C)C)CC=C(C)C)c(cc2O)O |
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| Description | Leachianone A, isolated from Radix Sophorae, has anti-malarial, anti-inflammatory, and cytotoxic potent[1]. Leachianone A induces apoptosis involved both extrinsic and intrinsic pathways[2]. |
| In Vitro | Leachianone A (0-20 μg/ml; 24-72 hours) exhibits a marked inhibition on the survival of HepG2 cells time- and dose-dependently manner, IC50 values are 6.9 μg/ml, 3.4 μg/ml and 2.8 μg/ml in cells with 24-, 48- and 72-hours treatment, respectively[1]. Leachianone A (10-30 μg/ml; 48 hours) indicates that at low concentration of LA (10 μg/ml), a substantial amount of cells is primarily in the early phase of apoptosis, at higher concentrations, induces a shift of the cell population to late apoptotic/ necrotic stage[1]. Leachianone A (10-30 μg/ml; 48 hours) decreases the precursor of caspase-3 in a dose-dependent manner, reduces the protein level of the pro-forms of upstream initiator caspases, caspases-8 and -9, decreases two downstream substrates, namely inhibitor of caspase-activated DNase(ICAD) and poly-ADP-ribose polymerase (PARP) in HepG2 cells[1]. Cell Viability Assay[1] Cell Line: HepG2 cells Concentration: 0 μg/ml, 2 μg/ml, 4 μg/ml, 6 μg/ml, 8 μg/ml, 10 μg/ml, 12 μg/ml, 14 μg/ml, 16 μg/ml, 18 μg/ml, 20 μg/ml Incubation Time: 24-72 hours Result: Inhibited HepG2 cells survival. Apoptosis Analysis[1] Cell Line: HepG2 cells Concentration: 48 hours Incubation Time: 10, 20, and 30 μg/ml Result: Induced the proportion of annexin V-stained cells in both the early and late apoptotic stage. Western Blot Analysis[1] Cell Line: HepG2 cells Concentration: 48 hours Incubation Time: 10, 20, and 30 μg/ml Result: Decreased the protein expression of caspase-3, caspases-8 and -9, reduced ICAD and PARP protein expression. |
| In Vivo | Leachianone A (intravenously injection; 20 mg/kg, 30 mg/kg; once daily; 30 days) significantly diminishes the tumor volume by 17-54% in LA-treated nude mice, when compared with those solely given the vehicle[1]. Animal Model: Male nude mice with human hepatoma HepG2 cells[1] Dosage: 20 mg/kg; 30 mg/kg Administration: Intravenously injection; 20 mg/kg, 30 mg/kg; once daily; 30 days Result: Suppressed the tumor growth in vivo. |
| Density | 1.2±0.1 g/cm3 |
| Boiling Point | 649.7±55.0 °C at 760 mmHg |
| Flash Point | 218.3±25.0 °C |
| Exact Mass | 438.204254 |
| PSA | 96.22000 |
| LogP | 7.27 |
| Vapour Pressure | 0.0±2.0 mmHg at 25°C |