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Brefeldin A
| Product Name | Brefeldin A |
| Price: | Inquiry |
| Catalog No.: | CN00257 |
| CAS No.: | 20350-15-6 |
| Molecular Formula: | C16H24O4 |
| Molecular Weight: | 280.36 g/mol |
| Purity: | >=98% |
| Type of Compound: | Alkaloids |
| Physical Desc.: | Powder |
| Source: | |
| Solvent: | Chloroform, Dichloromethane, Ethyl Acetate, DMSO, Acetone, etc. |
| SMILES: | O[C@@H]1C[C@@H]2C(C1)/C=C/CCC[C@@H](OC(=O)/C=C/[C@H]2O)C C16H24O4 |
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| Description | Brefeldin A is a specific inhibitor of protein trafficking which blocks the protein transport from the endoplasmic reticulum to the Golgi complex. |
| Target | CRISPR/Cas9 |
| In Vitro | Brefeldin A treatment for 15 h or 40 h, causes dramatic swelling of the Endoplasmic Reticulum (ER) and shifts its localization to the periphery of normal rat kidney (NRK) cells. Prolonged Brefeldin A treatment results in marked disruption of the MT and actin cytoskeleton[1]. ADP-ribosylation of BARS is mediated by formation of a conjugate between Brefeldin A and ADPR. BARS shows BAC binding when incubated with the medium from the BFA-treated CD38+ HeLa cells[2]. Brefeldin A induces anchorage-independent cell death in MDA-MB-231 breast cancer cells, inhibits the formation of MDA-MB-231 colonies in 3D and 2D cultures and inhibits the migration and MMP 9 (Matrix Metallopeptidase 9) activity of MDA-MB-231[3]. |
| Cell Assay | Cells are grown on glass coverslips, fixed in 3 % paraformaldehyde in PBS (10 min at room temperature) and then washed in PBS. Cells are permeabilized with 0.01 % Triton X-lOO in PBS at room temperature for 7 min. The coverslips are washed (3 times in PBS/0.2 % Tween) incubated in PBS/O.4 % fish skin gelatin/0.2 % Tween (5 min) and in PBS/2.5 % goat serum/0.2 % Tween (5 min.). After blocking, the cells are incubated with primary antibodies for 45 min at 37°C, and then washed with PBS/0.2 % Tween (5 times, 5 min each). The secondary antibodies are added for 30 min at 37°C and then cells are washed as above. Coverslips are mounted on slides in 9: 1 glycerol/PBS with 0.1 % o-phenylenediamine. |
| Density | 1.1±0.1 g/cm3 |
| Boiling Point | 492.7±45.0 °C at 760 mmHg |
| Flash Point | 180.8±22.2 °C |
| Exact Mass | 280.167450 |
| PSA | 66.76000 |
| LogP | 1.61 |
| Vapour Pressure | 0.0±2.8 mmHg at 25°C |
| Storage condition | 2~8°C |