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28-Deoxonimbolide
| Product Name | 28-Deoxonimbolide |
| Price: | Inquiry |
| Catalog No.: | CN07744 |
| CAS No.: | 126005-94-5 |
| Molecular Formula: | C27H32O6 |
| Molecular Weight: | 452.55 g/mol |
| Purity: | >=98% |
| Type of Compound: | Triterpenoids |
| Physical Desc.: | Powder |
| Source: | The barks of Azadirachta indica |
| Solvent: | Chloroform, Dichloromethane, Ethyl Acetate, DMSO, Acetone, etc. |
| SMILES: |
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| Description | 28-Deoxonimbolide is a nimbin (HY-N3187) type limonoid, that can be isolated from Azadirachta indica seed extracts. 28-Deoxonimbolide shows anticancer activity. 28-Deoxonimbolide induces apoptotic cell death in HL60 cells via both the mitochondrial- and the death receptor-mediated pathways[1]. |
| Target | Caspase-8 Caspase-9 Caspase-3 |
| In Vitro | 28-Deoxonimbolide (1-100 μM，48 小时) 对 HL60 (白血病)、A549 (肺)、AZ521 (胃)、SK-BR-3 (乳腺)、CRL1579 (黑色素瘤)和 RPMI1788 (正常淋巴细胞) 显示强有力的细胞毒性[1]。 28-Deoxonimbolide (30 μM, 0-24 h) 诱导 HL60 细胞凋亡[1]。 28-Deoxonimbolide (30 μM, 0-24 h) 可增加裂解的 caspases-8、-9 和 -3 的水平[1]。 Cell Cytotoxicity Assay[1] Cell Line: HL60, A549, AZ521, SK-BR-3, CRL1579 cells, RPMI1788 Concentration: 1-100 μM Incubation Time: 48 h Result: Exhibited potent cytotoxic activity against HL60 (leukemia), A549 (lung), AZ521 (stomach), SK-BR-3 (breast), and CRL1579 (melanoma) cells, with IC50 values of 2.7 μM, 9.3 μM, 2.4 μM, 1.7 μM, 14.2 μM, and 2.7 μM, respectively. Apoptosis Analysis[1] Cell Line: HL60 cells Concentration: 30 μM Incubation Time: 8 and 24 h Result: Induced early apoptosis in HL60 cells. The ratio of early apoptotic cells was increased after 8 h (10.9% vs 6.1% of negative control) and 24 h (18.7% vs 3.5% of negative control), and that of late apoptotic cells (upper right) was increased after 24 h (28.1% vs 2.9% of negative control). Western Blot Analysis[1] Cell Line: HL60 cells Concentration: 30 μM Incubation Time: 8 and 24 h Result: Diminished the levels of procaspases-8, -9, and -3, and then increased the levels of cleaved caspases-8, -9, and -3. |
| Density | 1.3±0.1 g/cm3 |
| Boiling Point | 554.2±50.0 °C at 760 mmHg |
| Flash Point | 289.0±30.1 °C |
| Exact Mass | 452.219879 |
| PSA | 74.97000 |
| LogP | 3.64 |
| Vapour Pressure | 0.0±1.5 mmHg at 25°C |